Novel Strategy for the Calorimetry-Based Control of Fed-Batch Cultivations of Saccharomyces cerevisiae

Typical controllers for fed-batch cultivations are based on the estimation and control of specific growth rate in real time. Biocalorimetry allows one to measure a heat signal proportional substrate consumed by cells. The derivative this is usually used evaluate rate, introducing noise resulting estimate. To avoid this, study investigated novel controller directly signal. Time trajectories setpoint were modelled different rates, was set follow dynamic setpoint. developed successfully followed during aerobic Saccharomyces cerevisiae, preventing Crabtree effect maintaining low glucose concentrations. With new method, S. cerevisiae could be reliably controlled at rates between 0.075 h?1 0.20 h?1, with average root mean square errors 15 ± 3%.